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019 _a1261305651
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020 _a9781839163524
_q(electronic book)
020 _a1839163526
_q(electronic book)
020 _a9781839163531
_q(electronic book)
020 _a1839163534
_q(electronic book)
020 _z9781839161636
020 _z1839161639
035 _a2970165
_b(N$T)
035 _a(OCoLC)1261761781
_z(OCoLC)1261305651
_z(OCoLC)1261364408
037 _a6203:6045
_bRoyal Society of Chemistry
_nhttp://www.rsc.org/spr
050 4 _aQP171
_b.A38 2021
082 0 4 _a572.4
_223
049 _aMAIN
245 0 0 _aAdvanced mass spectrometry-based analytical separation techniques for probing the polar metabolome /
_cedited by Rawi Ramautar.
264 1 _aCambridge :
_bRoyal Society of Chemistry,
_c2021.
300 _a1 online resource
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aNew developments in mass spectrometry ;
_vno. 10
504 _aIncludes bibliographical references and index.
588 0 _aOnline resource; title from digital title page (viewed on July 29, 2021).
505 0 _aIntro -- Title -- Copyright -- Contents -- Chapter 1 State-of-the-art in LC-MS Approaches for Probing the Polar Metabolome -- 1.1 Introduction: Metabolomics and the Polar Metabolome -- 1.2 LC Technologies: General Introduction -- 1.3 LC Technologies to Probe the Polar Metabolome -- 1.3.1 Hydrophilic Interaction Liquid Chromatography (HILIC) -- 1.3.2 Reversed-phase Liquid Chromatography (RPLC) with Derivatization -- 1.3.3 Ion-pair Chromatography (IPC) -- 1.3.4 Ion Exchange Chromatography (IEC) -- 1.3.5 Mixed-mode Chromatography (MMC) and Multimode Chromatography
505 8 _a1.3.6 Two-dimensional Liquid Chromatography (2D-LC) -- 1.4 Conclusions -- Abbreviations -- References -- Chapter 2 Chemical Derivatization for Polar Metabolome Analysis -- 2.1 Introduction -- 2.2 Chemical Derivatization LC-MS Analysis of Targeted Polar Metabolites -- 2.2.1 Amino Acid and Derivative Analysis -- 2.2.2 Nucleotide Analysis -- 2.2.3 Sugar Analysis -- 2.2.4 Small Organic Acid Analysis -- 2.3 Untargeted Analysis of Polar and Less-polar Metabolites -- 2.3.1 Main Features of CIL LC-MS -- 2.3.2 Data Processing in CIL LC-MS -- 2.3.3 Applications of CIL LC-MS -- 2.4 Concluding Remarks
505 8 _aAcknowledgements -- References -- Chapter 3 Ion Pair Liquid Chromatography-Mass Spectrometry for Probing the Polar Metabolome -- 3.1 Introduction -- 3.2 Ion Pair Liquid Chromatography (IP-LC) -- 3.2.1 Retention Mechanism of IP-LC -- 3.2.2 Critical Parameters of IP-LC -- 3.3 Mass Spectrometry -- 3.3.1 Targeted Analysis -- 3.3.2 Untargeted Analysis -- 3.4 Sample Preparation -- 3.4.1 Quenching -- 3.4.2 Extraction -- 3.4.3 Post-extraction -- 3.5 Ion Pair Liquid Chromatography-Mass Spectrometry-based Metabolomics -- 3.5.1 Central Carbon Metabolism -- 3.5.2 Amino Acids -- 3.5.3 Nucleotides
505 8 _a3.6 Potential Pitfalls in IP-LC-MS-based Metabolomics -- 3.6.1 Separation of Isomers -- 3.6.2 Ion Suppression -- 3.6.3 Detrimental Effect of Metal Cations -- 3.6.4 System Contamination -- References -- Chapter 4 Hydrophilic Interaction Chromatography-Mass Spectrometry (HILIC-MS) Approaches for Probing the Polar Metabolome -- 4.1 HILIC-MS: A Powerful Analytical Approach for the Qualitative and Quantitative Analysis of the Polar Cellular Metabolome -- 4.2 From Stationary Phases to Analytical Conditions and Retention Mechanisms -- 4.2.1 Stationary Phases According to Chemical Structure
505 8 _a4.2.2 Gradient Elution, Buffers and Ionic Strength of Mobile Phases -- 4.2.3 What is Known About Retention Mechanisms in HILIC -- 4.3 Selectivity, Sensitivity and Reproducibility of HILIC-MS approaches -- 4.4 Applications: From Single-pathway Targeted Quantification to Multiple Pathway Targeted and Untargeted Profiling -- 4.5 Isotopic Profiling -- 4.6 Conclusive Remarks -- Acknowledgements -- References -- Chapter 5 Advances in Solid Phase Microextraction (SPME) for Metabolomics -- 5.1 Introduction to Solid Phase Microextraction -- 5.2 Application of SPME in Foodomics
520 _aDiscussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field.
590 _aAdded to collection customer.56279.3
650 0 _aMetabolites
_xAnalysis.
_917400
650 0 _aMass spectrometry.
_917401
650 6 _aM�etabolites
_xAnalyse.
_917402
650 6 _aSpectrom�etrie de masse.
_917403
650 7 _amass spectrometry.
_2aat
_917404
650 7 _aMass spectrometry
_2fast
_917401
700 1 _aRamautar, Rawi,
_eeditor.
_917405
758 _ihas work:
_aAdvanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome (Work)
_1https://id.oclc.org/worldcat/entity/E39PD38GMF8VCPk87y9mJDxWCP
_4https://id.oclc.org/worldcat/ontology/hasWork
776 0 8 _iPrint version:
_tAdvanced mass spectrometry-based analytical separation techniques for probing the polar metabolome.
_dCambridge : Royal Society of Chemistry, 2021
_z9781839161636
_w(OCoLC)1255862364
830 0 _aNew developments in mass spectrometry ;
_v10.
_917406
856 4 0 _3EBSCOhost
_uhttps://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=2970165
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